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. 2012 Aug 21;18(2):243–249. doi: 10.1007/s12192-012-0367-x

Fig. 3.

Fig. 3

After downregulation of Hsp27, PTEN is increased and p-Akt is decreased. a In this case, PTEN was revealed using an affinity purified rabbit polyclonal antibody raised against a peptide mapping at the C-terminus of PTEN of human origin (1:400; PTEN C-20 sc-6817-R; Santa Cruz Biotech., Santa Cruz, CA, USA). 1 Untreated control MCF-7 cells; 2 control cells treated with Lipofectamine; 3 mock transfection control cells (72 h); 4 siHsp27-treated cells, 72 h after transfection. b Basal p-Akt levels (MW 70 kDa) in control MCF-7 cells (1) and in siHsp27-treated MCF-7 cells at 72 h after transfection (2). p-Akt was detected using a rabbit polyclonal antibody (1:1,000 dilution) generated against an epitope corresponding to amino acids 345–480 of Akt1 of human origin. This antibody detects Akt1, Akt2 and Akt3 (H-136, sc-8312, Santa Cruz Biotech., Santa Cruz, CA, USA). p-Akt was analyzed by Mann-Whitney test (graph)