Fig. 3.

Deletion and mutant analysis of the HSPA1A promoter. a Effects of PDT-induced oxidative stress on the luciferase activity of 5′deletion of the HSPA1A promoter. On the left was a schematic representation of the reporter gene constructs assayed by transient transfection of MCF-7 cells, while on the right side the bar graphs represented the relative levels of luciferase activity in each of the transfected samples. b Effects of PDT-induced oxidative stress on the mutations of the HSPA1A promoter. MCF-7 cells were transfected with different mutation HSPA1A promoter constructs or wild type HSPA1A promoter. Cells were then treated by Hyp-PDT with conditions described previously. The luciferase activity was measured 4 h post-PDT. Values are shown relative to the corresponding controls without PDT treatment. Columns, duplicate determinations representative experiment of three; bars, ±SD