Fig. 1.

The reduction-dependent aggregation of SOD1 in vitro. Wild-type (a) or G93A (b) SOD were incubated at 30 μM in 10 mM potassium phosphate buffer containing 5 mM EDTA, pH 7.4, whilst shaking (300 rpm for 5 min each cycle, 15-min cycles) at 37 °C in the absence or presence of 10 mM or 50 mM DTT. The buffer-alone sample is also shown and the concentration of DTT added is given on the right. The amount of ThT fluorescence (excitation at 440 nm and emission at 490 nm) was monitored over time using a microplate reader, and the change in ThT fluorescence is reported as a mean ± SEM of three replicates. The data are representatives of three independent experiments