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. 2013 Mar;141(3):335–345. doi: 10.1085/jgp.201210915

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© 2013 Li et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 5. — Stretch-induced effects on active force. A and C show original recordings of length and active force of 4 dpf control larvae stimulated during 200 ms with 200 Hz at optimal length. The contraction in A was isometric and the contraction in C involved a 10% stretch ramp (rate: 2 L_opt/s) imposed 60 ms after onset of activation. Muscle length was returned to optimal 60 ms after cessation of the stimulation. The force at 60 ms after activation (F₆₀), before the onset of the stretch ramp, and the maximal force (F_m) were evaluated. Force scale bar, 0.2 mN. The right panels show the contractile responses in muscles kept isometric (B, compare with A), or stretched (D, compare with C). F₆₀ (squares) and F_m (circles) values were normalized to the initial responses during the first contraction. The experiments were performed on control (open symbols, n = 11) and splicing morphants (closed symbols, n = 6). The force responses (F_m and F₆₀) of the splicing morphants were significantly (P < 0.001, one-way ANOVA and Holm-Sidak method) higher than the corresponding values in the control group during at the final 10th contraction of the stretch experiments (D). Error bars indicate mean ± SEM.