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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Genes Brain Behav. 2012 Nov 27;12(2):241–249. doi: 10.1111/j.1601-183X.2012.00867.x

Figure 1.

Figure 1

Targeted disruption of the GIR gene in the mouse: (a) Wild-type GIR gene is shown spanning exons 1–4. Lower line shows the transgene used to generate GIR −/− mice with targeted deletion of exons 2 and 3. ; Insertion cassette with position of flox (▶) sites, PGK-neo frt(▷) sites and genotyping primers F1, R1 and R2 is indicated. (b) Primers (F1) and (R1) produced a 438 bp band in the wild type GIR (+/+) and heterozygous (+/−) mice but not in GIR knockout (−/−) mice. Primers (F1) and (R2) produced a 484 bp band in (−/−) and (+/−) mice but a (~2.7kb) product in +/+ mice (not see here). (c) Reverse-transcriptase polymerase chain reaction revealed the absence of GIR message in various forebrain regions of (−/−) mice as compared to (+/+) mice. Lower panel shows Glial fibrillary acidic protein (GFAP) as loading control. PFC=prefrontal cortex, HYP=hypothalamus, HIP=hippocampus, STR=striatum.