Table 1.

Types and descriptions of morphological and motility related features used in classifying of instances of cell tracks into sessile vs. migratory states. Morphological features were computed based on the detected contour points of individual contour instance. Motility related features were computed based on the detected centroid across N intervals around the instance being feature computed. Step size is the one time step equivalent of cell speed and velocity autocorrelation function is the one time step equivalent of directional persistence.

Features	Notation	Type	Definition
step size	d	motility	centroid-to-centroid distance in subsequent time step
mean velocity autocorrelation function across N time step	μVACFN	motility	sample mean of dot products of N adjacent unit velocity vectors
variance velocity autocorrelation function across N time steps	σ2VACFN	motility	sample variance of dot products of N adjacent unit velocity vectors
cell size	A	morphological	area enclosed by cell contour
cell elongation	ℓ	morphological	ratio between cell major and minor axes
cell orientation	θ	morphological	dot product of unit instantaneous velocity vector and unit major axis