A) Relaxed swelling ratio of PEGDA hydrogels with different molecular weight at different macromer concentrations in the initial solution. Swelling ratio increases with increasing molecular weight and increasing macromer concentration. No swelling is indicated by the dashed line, where QR = 1.0. Insert: AFM images of 4000 nm pitch topography showing the deformation of the topographic features in hydrogels due to swelling.
B) Cell attachment to unfunctionalized 20% (w/w) PEGDA hydrogels synthesized with macromers of molecular weight 700 g/mol and 3400 g/mol using EP media. PEGDA 3400 exhibits less non-specific cell attachment to the surfaces than PEGDA 700.