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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: J Biomed Mater Res A. 2012 Sep 11;101(4):1069–1079. doi: 10.1002/jbm.a.34417

Figure 3.

Figure 3

A) Net orientation of cells and nuclei when cultured in EpiLife and EP media. Net orientation was determined as the difference between total number of objects aligned parallel to those aligned perpendicular to the substratum. Nuclei of cells cultured on the 400 nm pitch in EpiLife medium were predominantly net-perpendicular (~10%) while their cell bodies were aligned net-parallel (~15%). However, in EP medium both cell and nuclei had a net parallel orientation to the topography. Results are Mean of n=8 individual experiments. B) Representative images of primary corneal epitheial cells aligned (i) perpendicular to the underlying surface (800 nm) and (ii) parallel to the topography (1200 nm).