Fig. 2.

RP chromatogram obtained from the mAb glycan standard showing the grouping of the eluting 2-AA N-glycans. (A) High-mannose structures elute first (green), followed by non-fucosylated hybrid (orange) and complex glycans (blue). Fucosylated hybrid (orange) and complex structures (blue) elute last in the chromatogram. Acidic glycans elute immediately before their appropriate neutral glycans (pink). (B) Magnified view of the region between 18 and 42 min showing the less abundant glycans. The identified glycans are numbered and the appropriate masses are listed in Table 1. Stacked numbering indicates co-elution of N-glycans. The glycan structures are depicted in Fig. 3