Table 1.
2-AA and 2-AB-labeled glycans from mAb glycan standard identified by ion-trap MS and MS2. Observed and theoretical mass are shown for each assigned glycan. For N-glycan isomers only one mass is shown. The peak numbers correspond to the appropriate peak numbering in the chromatograms and to the structures in Fig. 3
| Peak | 2-AA Glycans | 2-AB Glycans | N-Glycan | ||
|---|---|---|---|---|---|
| Observed mass | Theoretical mass | Observed mass | Theoretical mass | ||
| 5 | 1841.602 | 1841.645 | 1840.689 | 1840.661 | M8 |
| 6, 7, 8, 9 | 1679.541 | 1679.592 | 1678.604 | 1678.608 | M7 |
| 10 | 1031.406 | 1031.381 | 1030.425 | 1030.397 | M3 |
| 11 | 1234.470 | 1234.460 | 1233.489 | 1233.476 | M3G0 |
| 12 | 2027.716 | 2027.709 | 2026.739 | 2026.725 | SM5G1 |
| 13, 14 | 1517.592 | 1517.539 | 1516.573 | 1516.555 | M6 |
| 15 | 1396.550 | 1396.513 | 1395.54 | 1395.529 | M3G1 |
| 16 | 1855.655 | 1865.656 | 1865.678 | 1864.672 | SM4G1 |
| 17, 18, 19 | 1703.619 | 1703.603 | 1702.615 | 1702.619 | SM3G1 |
| 20, 21 | 1193.457 | 1193.433 | 1192.49 | 1192.449 | M4 |
| 22 | 1355.525 | 1355.486 | 1354.533 | 1354.502 | M5 |
| 23, 24 | 1558.617 | 1558.566 | 1557.582 | 1557.582 | M5G0 |
| 25 | 1720.647 | 1720.618 | 1719.643 | 1719.634 | M5G1 |
| 26, 27, 28, 29 | 1599.618 | 1599.592 | 1598.612 | 1598.608 | G1 |
| 30 | 1720.647 | 1720.618 | 1719.643 | 1719.634 | M6G0 |
| 31 | 1558.592 | 1558.566 | 1557.576 | 1557.582 | M4G1 |
| 32, 33 | 1437.586 | 1437.539 | 1436.565 | 1436.555 | G0 |
| 34 | 1687.579 | 1687.608 | – | 1686.624 | SM3G1 |
| 35, 36 | 1786.675 | 1786.677 | 1785.708 | 1785.693 | G0F + GN |
| 37 | 1583.710 | 1583.597 | 1582.648 | 1582.613 | G0F |
| 38 | 2173.726 | 2173.767 | 2172.78 | 2172.783 | SM5G1F |
| 39 | 2011.693 | 2011.714 | 2010.754 | 2010.73 | SM4G1F |
| 40 | 2376.763 | 2376.846 | 2375.787 | 2375.862 | SG3F |
| 41 | 1849.705 | 1849.661 | 1848.692 | 1848.677 | SM3G1F |
| 42, 43 | 2214.754 | 2214.793 | 2213.818 | 2213.809 | SG2F |
| 44, 45 | 2052.732 | 2052.740 | 2051.768 | 2051.756 | SG1F |
| 46 | 1948.741 | 1948.729 | 1947.804 | 1947.745 | G1F + NG |
| 47 | 2028.702 | 2028.729 | – | 2027.745 | M6G1F |
| 48, 49 | 1907.689 | 1907.703 | 1906.775 | 1906.719 | G2F |
| 50 | 1866.679 | 1866.676 | 1865.698 | 1865.692 | M5G1F |
| 51, 52 | 1704.609 | 1704.624 | 1703.644 | 1703.64 | M5G0F |
| 53 | 1866.650 | 1866.676 | 1865.668 | 1865.692 | M6G0F |
| 54 | 1583.533 | 1583.597 | 1582.608 | 1582.613 | M3G0F + NG |
| 55 | 1704.609 | 1704.624 | 1703.643 | 1703.64 | M4G1F |
| 56, 57 | 1542.566 | 1542.571 | 1541.582 | 1541.587 | M3G1F |
| 58 | 2069.748 | 2069.756 | – | 2068.772 | G3F |
| 59 | 1380.553 | 1380.518 | 1379.569 | 1379.534 | M3G0F |
| 60 | 1907.690 | 1907.703 | 1906.746 | 1906.719 | G2F |
| 61, 62 | 1745.610 | 1745.650 | 1745.655 | 1744.666 | G1F |
| 63 | 1583.709 | 1583.597 | 1582.607 | 1582.613 | G0F |