Table 5.
The effect of NAM treatment (NAM/vehicle) and TBI (vehicle/sham) on differentially expressed genes associated with significant functional categories and canonical pathways identified by IPA at 24 h post-TBI.
| Affymetrix ID | Gene symbol | Genes | 24 h post-TBI |
72 h post-TBI |
||
|---|---|---|---|---|---|---|
| NAM/vehicle | Vehicle/sham | NAM/vehicle | Vehicle/sham | |||
| 10802691 | Acaa2 | Acetyl-Coenzyme A acyltransferase 2 | n.s. | n.s. | 0.66 | 2.29 |
| 10742213 | Adra1b | Adrenergic, alpha-1B-, receptor | n.s. | n.s. | 1.53 | 0.49 |
| 10811900 | Agt | Angiotensinogen (serpin peptidase inhibitor, clade A, member 8) | n.s. | 0.55 | 0.66 | n.s. |
| 10767723 | Atp2b4 | ATPase, Ca++ transporting, plasma membrane 4 | n.s. | n.s. | 1.61 | 0.53 |
| 10746327 | Cacna1g | Calcium channel, voltage-dependent, T type, alpha 1G subunit | n.s. | n.s. | 1.56 | 0.57 |
| 10845306 | Cacnb4 | Calcium channel, voltage-dependent, beta 4 subunit | n.s | n.s. | 1.55 | 0.43 |
| 10921030 | Cck | Cholecystokinin | n.s. | n.s. | 2.27 | 0.38 |
| 10763401 | Cdh7 | Cadherin 7, type 2 | n.s. | n.s. | 1.55 | 0.38 |
| 10813858 | Cdh12 | Cadherin 12 | n.s. | 0.64 | 1.89 | 0.26 |
| 10910133 | Chrna5 | Cholinergic receptor, nicotinic, alpha 5 | n.s. | n.s. | 3.22 | n.s. |
| 10926642 | Clic5 | Chloride intracellular channel 5 | n.s. | n.s. | 1.52 | n.s. |
| 10736697 | Ccl2* | Chemokine (C-C motif) ligand 2 | n.s. | 44.15 | n.s. | 36.50 |
| 10930204 | Efna5 | Ephrin A5 | n.s. | n.s. | 2.28 | 0.27 |
| 10781337 | Egr3 | Early growth response 3 | n.s. | n.s. | 1.61 | 0.51 |
| 10854961 | Ephb6 | Eph receptor B6 | 3.09 | n.s. | 1.63 | 0.50 |
| 10909987 | Exph5 | Exophilin 5 | n.s. | n.s. | 1.60 | 0.31 |
| 10940090 | Gabra3 | Gamma-aminobutyric acid (GABA) A receptor, alpha 3 | n.s. | n.s. | 1.56 | 0.46 |
| 10935811 | Gabre | Gamma-aminobutyric acid (GABA) A receptor, epsilon | n.s. | 1.52 | 1.50 | n.s. |
| 10818306 | Gnat2 | Guanine nucleotide binding protein, alpha transducing 2 | n.s. | n.s. | 0.61 | 1.81 |
| 10733680 | Gpx3 | Glutathione peroxidase | n.s. | n.s. | 0.67 | 2.21 |
| 10871939 | Grik3 | Glutamate receptor, ionotropic, kainite 3 | n.s. | n.s. | 1.67 | 0.47 |
| 10828351 | HLA-DQA1 | Major histocompatibility complex, class II, DQ alpha 1 | n.s | 1.65 | 0.62 | 3.58 |
| 10852258 | Hrh3 | Histamine receptor H3 | n.s. | n.s. | 1.58 | 0.54 |
| 10752663 | Htr1f | 5-Hydroxytryptamine (serotonin) receptor 1F | n.s. | 0.66 | 1.59 | 0.39 |
| 10781467 | Htr2a | 5-Hydroxytryptamine (serotonin) receptor 2A | n.s. | n.s. | 2.37 | 0.31 |
| 10889326 | Kcnf1 | Potassium voltage-gated channel, subfamily F, member 1 | n.s. | n.s. | 1.68 | 0.33 |
| 10888131 | Kcng3 | Potassium voltage-gated channel, subfamily G, member 3 | n.s. | n.s. | 1.52 | 0.57 |
| 10845725 | Kcnh7 | Potassium voltage-gated channel, subfamily H (eag-related), member 7 | n.s. | 0.65 | 1.51 | 0.34 |
| 10849655 | Kcnip3 | Kv channel interacting protein 3, calsenilin | n.s. | n.s. | 2.94 | n.s. |
| 10724895 | Lyve1 | Lymphatic vessel endothelial hyaluronan receptor 1 | n.s. | n.s. | 0.58 | 3.37 |
| 10820223 | Mef2c | Myocyte enhancer factor 2C | n.s. | n.s. | 1.78 | 0.38 |
| 10907913 | Mmp8* | Matrix metallopeptidase 8 | n.s. | 3.24 | n.s. | 4.15 |
| 10842239 | Mmp9* | Matrix metallopeptidase 9 | n.s. | 3.36 | 1.60 | n.s. |
| 10795077 | Nrsn1* | Neurensin 1 | n.s. | n.s. | 2.99 | 0.39 |
| 10782986 | Otx2 | Orthodenticle homeobox 2 | n.s. | n.s. | 0.41 | 3.37 |
| 10846694 | Ped1a | Phosphodiesterase 1A, calmodulin-dependent | n.s. | n.s. | 3.68 | 0.44 |
| 10795989 | Pfkp | Phosphofructokinase, platelet | n.s. | n.s. | 1.70 | 0.59 |
| 10764551 | Ptgs2* | Prostaglandin-endoperoxide synthase 2 | n.s. | 1.85 | 1.72 | n.s. |
| 10846694 | Pde1a | Phosphodiesterase 1A, calmodulin-dependent | n.s. | n.s. | 1.88 | 0.44 |
| 10744641 | Pitpnm3 | PITPNM family member 3 | n.s. | n.s. | 1.57 | 0.48 |
| 10769672 | Rgs4 | Regulator of G-protein signaling 4 | n.s. | n.s. | 1.82 | 0.30 |
| 10827686 | RT1-M6-1 | RT1 class I, locus M6, gene 1 | n.s. | n.s. | 2.16 | 0.50 |
| 10827691 | RT1-M6-2 | RT1 class I, locus M6, gene 2 | n.s. | n.s. | 1.89 | 0.62 |
| 10759923 | Rxfp2 | Relaxin/insulin-like family peptide receptor 2 | n.s. | 0.66 | 1.51 | 0.65 |
| 10836458 | Scn2a | Sodium channel, voltage-gated, type II, alpha subunit | n.s. | n.s | 1.50 | 0.46 |
| 10921274 | Satb1 | SATB homeobox 1 | n.s. | n.s. | 1.52 | 0.42 |
| 10928191 | Satb2 | SATB homeobox 2 | n.s. | n.s. | 2.50 | 0.24 |
| 10849943 | Slc4a11 | Solute carrier family 4, sodium bicarbonate transporter-like, member 11 | n.s. | n.s. | 1.56 | 0.65 |
| 10751945 | Sst | Somatostatin | n.s. | n.s. | 1.57 | 0.49 |
| 10884732 | Sstr1 | Somatostatin receptor 1 | n.s. | n.s. | 1.64 | 0.43 |
| 10930711 | St6gal2 | ST6 beta-galactosamide α-2,6-siayltransferase 2 | n.s. | 0.66 | 1.81 | 0.45 |
| 10802795 | St8sia5 | ST8 alpha-N-acetyl-neuraminide α-2,8-sialyltransferase 5 | n.s. | n.s. | 1.61 | 0.44 |
| 10763171 | Stx1a | Syntaxin 1A (brain) | n.s. | n.s. | 2.16 | 0.32 |
| 10722830 | Sv2b | Synaptic vesicle glycoprotein 2b | n.s. | n.s. | 1.81 | 0.43 |
| 10819139 | Tacr3 | Tachykinin receptor 3 | n.s. | 0.50 | 1.59 | 0.29 |
| 10845628 | Tbr1 | T-box, brain, 1 | n.s. | n.s. | 1.90 | 0.32 |
| 10722097 | Tph1 | Tryptophan hydroxylase 1 | n.s. | n.s. | 0.47 | 2.23 |
Results are given as fold change with significance defined as 1.5-fold change, p < 0.05. The degree of differential expression of these genes is also shown for the NAM/Vehicle and Vehicle/Sham contrasts for the 24-h post-TBI time point.
*RT-PCR validated (see Figure 6).
n.s., not significant.