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Recognition of MAGE-A3-like peptides. The human genome was searched for peptide similar to MAGE-A3 that were predicted to have physiologically relevant HLA-A*0201 binding affinities and were expressed in human brain. These eight peptides were synthesized and pulsed onto T2 cells along with MAGE-A3 and MAGE-A12 peptides at the indicated concentrations. Shown is the resultant gamma-interferon (IFN-γ) production (pg/ml) following overnight co-culture.
