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. 2013 Feb 26;3:1345. doi: 10.1038/srep01345

Figure 4. Electrophysiological properties of iNPC-derived neurons assessed by whole-cell patch-clamp recordings.

Figure 4

A phase contrast image of a patch pipette attached to the membrane of a cultured iNPC-differentiated neuron (A). Typical traces of action potential-like spike induced by current injection steps before (B) and after (C) 1 μM TTX treatment. (D) A-type K+ currents were induced by an initial hyperpolarization (voltage steps from the holding potential of −60 mV to −90 mV and return to −60 mV in the first step), and then stepped to +30 mV in increments of 10 mV (500 ms duration) in the presence of 10 mM TEA. (E) D-type K+ currents were induced by an initial depolarization (voltage steps from the holding potential of −60 mV to −30 mV and return to −60 mV in the first step), and then stepped to +30 mV in increments of 10 mV (500 ms duration) in the presence of 1 mM 4-aminopyridine. Bar graphs show the resting membrane potentials (F) and membrane capacitances (G) of iNPC-derived neurons. (Scale bars: 50 μm).