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. 2013 Jan 16;33(3):1050–1061. doi: 10.1523/JNEUROSCI.1704-12.2013

Figure 4.

Figure 4.

Target-derived BMP4 promotes sensory neurite outgrowth. A, Dissociated DRG neurons grown on collagen-coated plates showed increased neurite outgrowth in the presence of 10 or 50 ng/ml BMP4 (*p ≤ 0.007). B, Vaginal smooth muscle cells immunostained for α-smooth muscle actin (red) were grown on collagen-coated plastic. DRG neurons immunostained for peripherin (green) were plated on smooth muscle cells, and most extended neurites that made contacts with muscle cells at 48 h. C, Vehicle-treated cocultures immunostained for peripherin showed many neurites. D, Cocultures treated with noggin (250 ng/ml) had fewer neurites. E, Quantitative analysis confirmed a reduction in neurite outgrowth per neuron in noggin-treated cocultures (**p = 0.001). F, Neurites elaborated by neurons grown directly on smooth muscle cocultures (Control) are reduced by the addition of a BMP4 function-neutralizing antibody (#p = 0.005; G, H). Anti-BMP4 was equally effective in reducing axon outgrowth when smooth muscle cells were grown in transwell inserts (H, Control-T, Anti-BMP4-T; ##p = 0.004). Scale bars, 50 μm.