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. 2013 Feb 6;110(8):2969–2974. doi: 10.1073/pnas.1222694110

Fig. 5.

Fig. 5.

DMXAA-mediated gene expression requires STING but not MRE11. (A) Immunofluorescence staining of stably expressed STING-HA in ATLD2SV cells stimulated with 10 μM cisplatin for 24 h. (B) qPCR analysis of mouse Ifnb1, Cxcl10, and Pml in wild-type and Sting−/− MEFs treated with 50 μg⋅mL−1 DMXAA for 4 h. (C) TUNEL assay in wild-type and Sting−/− MEFs treated with 50 μg⋅mL−1 DMXAA for 12 h. Data are from one experiment representative of two. (D) Immunofluorescence staining of stably expressed STING-HA in ATLD2SV cells stimulated with 100 μg⋅mL−1 DMXAA for 1 h. Data are from one experiment representative of two. (E) qPCR analysis of mouse Ifnb1 in MEFs treated with a control siRNA or siRNAs (mM11-3 or mR50-1) and stimulated with 100 μg⋅mL−1 DMXAA for 4 h. Results for mRNA are represented relative to those of control samples. Data are from three independent experiments in triplicate (mean and SD in B and E). *P < 0.05 and **P < 0.005 compared with controls.