Skip to main content
. 2013 Feb 4;110(8):3173–3178. doi: 10.1073/pnas.1219229110

Fig. 1.

Fig. 1.

Hsp90C is associated with preprotein import intermediates. (A) Diagrams of the preproteins used in this study. (B) Urea-denatured preatSSU–3xFLAG and preatTic40–3xFLAG were incubated with isolated pea chloroplasts under import conditions. After the reaction, the chloroplasts were isolated and treated with 1 mM DSP in the dark for 15 min. Chloroplasts (1 mg of chlorophyll) were dissolved in buffer containing 1% Triton X-100 and immunoprecipitated with M2 Flag antibody. Equivalent fractions of total chloroplast detergent extract (L), unbound material (UB), and immunoaffinity eluates (E), were resolved by SDS/PAGE and the resolved polypeptides were visualized by immunoblotting with antisera to chloroplast proteins as indicated to the Left of each panel. Lanes 4 and 8 of the Lower panels contain 10% of the preprotein added to each import reaction (Pr). Gels in the Lower panels were loaded with 10% of the material in the Upper panels and were probed with M2 FLAG antibody to detect preprotein intermediates.