Fig. 4.

NES in S. aureus and polyamide inhibition. (A) Effect of nes deletion (KO) and complementation with WT and designed NES loop 1 (L1), loop 2 (L2), and C-terminal deletion (ΔC-term) mutants on conjugation in S. aureus. (B) Structures of the Match Polyamide 1 and Mismatch Polyamide 2. (C) Inhibition of full-length NES with Match 1 (solid bars) and Mismatch 2 (shaded bars) polyamides. (D) Eighteen- and 21-μM IC₅₀ values of the Match Polyamide 1 for the full-length and relaxase forms of NES, respectively.