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. 2013 Feb 4;110(8):3161–3166. doi: 10.1073/pnas.1222051110

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Fig. 2. — Bcl-6 expression is enhanced by AMPK phosphorylation of PARP-1. (A and B) HUVECs were transfected with Bcl-6-Luc or Bcl-6-Luc(Δ) constructs, then treated with AICAR, metformin (met), or metformin + Compound C for luciferase assay or ChIP using anti-PARP-1 and primers for Bcl-6-Luc or Bcl-6-Luc(Δ). (C) HUVECs transfected with Bcl-6-Luc, infected with Ad-AMPK-CA or Ad-AMPK-DN, and cotransfected with S177A or S177D for luciferase activity. (D–F) Bcl-6 mRNA levels in HUVECs transfected with control, AMPKα, or PARP-1 siRNA, then treated with met or AICAR. (G) Bcl-6 expression in HUVECs treated with metformin or metformin + Compound C. (H) Bcl-6 expression in HUVECs transfected with control, AMPKα siRNA, or PARP-1 siRNA, then treated with AICAR. (I) Bcl-6 and Flag-PARP-1 expression in HUVECs transfected with PARP-1, S177A, or S177D, then treated with AICAR or AICAR + Compound C. *P < 0.05.