Fig. 2.

(A) The post-SCI chronic development of above-injury-level and below-injury-level hypersensitivity was evaluated with a modified von Frey filament test, as diagrammed. The forepaw- and hindpaw-withdrawal thresholds to von Frey filaments of several stiffnesses were tested in acclimated and resting conscious rats. The paw area probed by the filament is shaded in gray and indicated by the long arrow. (B) Baseline data of changes in the paw-withdrawal threshold over the study’s time course. (C and D) The effect is dose dependent. I.p. injection of varying doses of HUP-A resulted in reproducible anti-hypersensitivity effects, i.e., increased withdrawal thresholds of the forepaws (C) and hindpaws (D), in the chronic stage of SCI (i.e., 4 wk after injury). Sal, saline.