Fig. 6.

Early and sustained increase in deep layer genesis and delayed upper layer genesis following sustained Ikaros expression in the cortex. (A–D) Immunostaining and quantifications of Tbr1 (layer 6, red), Ctip2 (weak in layer 6 and strong in layer 5, green), and Satb2 (layers 2–4; blue) in WT and D6-Ikaros lateral cortex at different stages of cortical development as indicated. (E–H) Immunostaining and quantifications of the same markers in WT and D6-Ikaros medial cortex. Increased numbers of Tbr1⁺ and Ctip2⁺ deep layer neurons were observed in D6-Ikaros compared with WT littermates throughout development both laterally and medially, as well as a delayed appearance and decreased numbers of Satb2⁺ upper layer neurons throughout development. In B–D and F–H, values represent mean ± SEM (n = 3–7 brains). *P < 0.05; **P < 0.01; ***P < 0.001. (Scale bars: 50 μm.)