Fig. 8.

(A and B) TIRFM detection of (A) BODIPY-sphingolipids (green) and (B) influenza hemagglutinin (red) in the plasma membrane of a living Clone 15 cell at 37 °C. (C) Overlay of the two images shows colocalization between the sphingolipids and hemagglutinin, denoted by the yellow color. Stacks of 120 frames were collected for each channel and averaged to increase signal-to-noise ratio. Final images are shown at 2× magnification without interpolation. The influenza hemagglutinin and sphingolipid domains were partially colocalized.