Fig. 2.
Kissing between a pair of adjacent mitochondria. (A) Confocal images of interfibrillar mitochondria in cardiomyocytes expressing mtPAGFP and stained with TMRM, at indicated time points after photoactivation. (Scale bar: 2 μm.) (B) Time courses of mtPAGFP fluorescence in adjacent mitochondria, mito-1 and mito-2. (C) As in B, for TMRM fluorescence. (D) Spatial profiles showing the mtPAGFP fluorescence intensity along the mitochondrial bundle immediately or 10 min after photoactivation. (E) Space–time visualization of a mitochondrial kissing event. Time runs from top to bottom and mitochondrial location is shown horizontally. (F) Normalized mtPAGFP fluorescence intensity in donor mitochondria before and after kissing. (G) Mitochondrial transfer index.