Fig. 5.

Icm/Dot-dependent translocation of Legionella effector proteins predicted by the HSMM. CyaA fusions of predicted L. pneumophila (A) and L. longbeachae (B) effectors were examined for translocation into HL-60–derived human macrophages from the WT strain JR32 (gray bars) and the icmT deletion mutant GS3011 (white bars); the effectors examined are indicated below each bar. V, vector control. The cAMP levels of the infected cells were determined as described in Materials and Methods. The bar heights represent the means of the amount of cAMP per well obtained in at least three independent experiments; error bars indicate SDs. The cAMP levels of each fusion were found to be significantly different (*P < 0.05; **P < 0.01; ***P < 0.001, paired Student t test) between the WT strain and the icmT deletion mutant.