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. 2013 Feb 5;110(8):2864–2869. doi: 10.1073/pnas.1212484110

Fig. 2.

Fig. 2.

ER stress in goblet cells of IRE1β−/− mice. (A) The ratio of spliced to unspliced XBP1 mRNA is elevated in IRE1β−/− colon but decreased in IRE1α−/− colon. RNAs were extracted from two individual colons of wild-type (IRE1β+/+, IRE1α+/+), IRE1β−/−, IRE1α+/−, or IRE1α−/− mice. After RT-PCR, the intensity of each band was measured, and the ratio (s/u) of spliced to unspliced XBP1 mRNA was calculated. (B) Increase of BiP in IRE1β−/− colons. Western blotting was performed with anti-BiP antibody for colon lysates from wild-type (IRE1β+/+, IRE1α+/+), IRE1β−/−, IRE1α+/−, or IRE1α−/− mice. Signal intensity was normalized using β-actin, and fold induction relative to the level for the IRE1β+/+ sample was calculated. (C) Increase of BiP in IRE1β−/− goblet cells. Cryosections of IRE1β+/+ or IRE1β−/− colons were stained with anti-BiP (green) antibody and DAPI (blue). Double-headed arrow indicates the region where BiP-induced goblet cells were prominent. The “+ DIC” image depicts superimposed immunofluorescence and DIC images.