Skip to main content
. 2013 Feb 10;2013:748687. doi: 10.1155/2013/748687

Figure 1.

Figure 1

Fermented antler extract inhibits RANKL-induced osteoclast differentiation. (a) BMM cells were cultured for 4 days in the presence of RANKL (5 ng/mL) and M-CSF (30 ng/mL) with antler or fermented antler extract. Osteoclasts were visualized by TRAP staining. (b) The number of TRAP-positive osteoclasts (TRAP+OCs) was counted. ***P < 0.001 (versus “the control”). (c) TRAP activity was measured. ### P < 0.001 (versus “the negative control”); ***P < 0.001 (versus “the group treated with RANKL alone”). (d) The effect of fermented antler extract on the viability of BMMs was evaluated by CCK-8 assay. Each experiment was performed in triplicate. Statistical differences were analyzed with the Student's t-test and all quantitative values were presented as mean ± SD.