Figure 5.

Immunolocalization of NHE2 in steroid replaced ovariectomized rats and in rats at different phases of the oestrous cycle. NHE2 was expressed at both the apical and basolateral membrane of the endometrial epithelia. Its expression was increased under E-dominance, however was reduced under P dominance. Magnifications of 10X and 100X (in the upper right corner). 0.2E: 0.2 μg 17β-oestradiol, 2E: 2 μg 17β-oestradiol, 20E: 20 μg 17β-oestradiol, 50E: 50 μg 17β-oestradiol, P: progesterone, E + P: 0.2 μg 17β-oestradiol + progesterone. Ps: proestrus, Es: estrus, Ms: metestrus, Ds: diestrus. n = 6 rats per group. Incubation with a non-immune peptide was used as a negative control to check for the specificity of the antibodies. No staining was observed in this experiment.