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. 2013 Feb 11;2013:840121. doi: 10.1155/2013/840121

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Copyright © 2013 Khadijeh Gholami et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Immunolocalization of NHE4 in steroid replaced ovariectomized rats and in rats at different phases of the oestrous cycle. Bilateral distribution of NHE4 was observed following E treatment and at Ps and Es stages of the cycle, however the expression was reduced under P dominance. Magnifications of 10X and 100X (in the upper right corner). 0.2E: 0.2 μg 17β-oestradiol, 2E: 2 μg 17β-oestradiol, 20E: 20 μg 17β-oestradiol, 50E: 50 μg 17β-oestradiol, P: progesterone, E + P: 0.2 μg 17β-oestradiol + progesterone. Ps: proestrus, Es: estrus, Ms: metestrus, Ds: diestrus. n = 6 rats per group. Incubation with a non-immune peptide was used as a negative control to check for the specificity of the antibodies. No staining was observed in this experiment.