Figure 4. SPRY2 deficiency induces TP53-dependent G₁ arrest via nuclear PTEN.

(A) Western blot analysis of indicated DU145 cells transfected with PTEN siRNA. (B) Western blot analysis of PTEN IP from DU145. (C) Western blot analysis for LNCaP cells transfected with indicated plasmids. (D) DU145 cells transfected with TP53 siRNA were analyzed by Western blot and quantified for cells in G₁. (*P < 0.01; n = 3, analyzed by Mann-Whitney test). Data are presented as mean ± SEM. (E) Representative IHC images for Srpy2, Trp53, and p21 in prostates of mice as indicated (n = 4). Scale bars: 50 μm. All Western blots were quantified using ImageJ, and the values represent relative immunoreactivity of each protein normalized to respective loading control.