Figure 4. Classical IL-6 signaling and IL-6 trans-signaling activate different pathways in the pancreas during inflammation.

At 0, 4, and 8 hours, pancreatic tissue from C57BL/6, Il6^–/–, and opt_sgp130Fc mice was isolated and homogenized to detect p-STAT3^Y705, p-STAT3^S727, p-RelA, IκBα, and IκBβ. ERK1/2 served as loading control (representative blot; n = 4 per time point).