Figure 3. MFGE8 inhibits the inflammasome pathway via β₃ integrin.

(A) Representative photomicrographs of costaining for P2RX7 and β₃ integrin in BMDM before and after LPS priming and analysis using confocal microscopy. A tight spatial association (quantified by the overlap coefficient) was observed between β₃ integrin and P2RX7 after LPS priming. (B) Duolink in situ PLA colocalization assay proving the spatial association between β₃ integrin and P2RX7 in BMDM after LPS priming. The spots in B indicate colocalized P2X7R and β₃. Data are representative of 3 independent experiments. (C) MFGE8 did not inhibit ATP-induced IL-1β production or caspase-1 activity in BMDM recovered from Itgb3^–/– mice. (D and E) Quantification of infarct volume in Itgb3^–/– and P2rx7^–/– mice treated or not with rMFGE8; rMFGE8 did not reduce infarct volume in Itgb3^–/– and P2rx7^–/– mice. **P < 0.01; ***P < 0.001; n = 6 mice per group for in vivo experiments. Scale bars: 10 μm.