Skip to main content
. 2013 Feb 15;123(3):1057–1067. doi: 10.1172/JCI65344

Figure 6. HRM/AGO1 pathway regulates hypoxia-induced angiogenesis in vivo.

Figure 6

(A–C) Matrigel mixed with control RNA (15 μg/plug) or antagomirs against Let-7a, Let-7e, and miR-103 (5 μg/plug each) was injected s.c. into C57BL/6 mice before 5-day normoxia or hypoxia. Gross morphology (A) and Hb quantification (B) as well as H&E, vWF, and CD31 staining of Matrigel plugs (C). (D and E) HUVECs transfected with control or HA-AGO1-ORF plasmid were mixed with Matrigel and s.c. injected into SCID mice, which were then kept under normoxia or hypoxia for 3 days. Gross morphology (D) was photographed and Hb quantified (E). (F–H) HUVECs transfected with control RNA (40 nM) or AGO1 siRNA (20 or 40 nM) were mixed with Matrigel and s.c. injected into SCID mice. Five days after injection, the plugs were harvested for gross morphology (F), Hb quantification (G), histology, and IHC (H). In C, E, and G, the Hb content in normoxia/control RNA or control plasmid group was set to 1. Bar graphs represent mean ± SEM. Results are from 5–10 animals per group. Scale bar: 100 μm. *P < 0.05 compared with controls or between indicated groups.