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Dkk-3 deters invasive capacity of OS cells via downregulation of MMP-2 and -9 activities. (a) Gelatinolytic activities of MMP-2 and MMP-9 for Dkk-3-transfected SaOS-2 OS cells compared to vector control were evaluated by zymographic analysis. (b) Representative photographs of immunohistochemical detection of MMP-2 of pulmonary metastatic nodules in inoculated mice with Dkk-3-transfected-143B OS; magnification 400x.
