Figure 10.

The development of anti-α3(IV)NC1 autoantibodies in DRB1*15:01 Tg or DRB1*01:01 Tg mice deficient in FcγRIIb (1501 Tg^{.FcγRIIb−/−} and 0101 Tg^{.FcγRIIb−/−}, respectively). (A) Sera from α3_136–146 immunized and control mice (n=8 per group) are assayed for anti-murine α3(IV)NC1 antibodies, which are present to a similar titer in both strains of mice. However, only α3_136–146 immunized 1501 Tg^{.FcγRIIb−/−} mice developed IgG deposition in glomeruli (B), which when assessed by direct immunofluorescence is not present in α3_136–146 immunized 0101 Tg^{.FcγRIIb−/−} mice or nonimmunized mice (ctrl) of either strain. (C) IgG deposition is observed in glomeruli of all 8 α3_136–146 immunized 1501 Tg^{.FcγRIIb−/−} mice. Direct immunofluorescence reveals IgG staining of the glomerular basement membrane in a linear pattern with granular components in all mice (×1000). ***P<0.001.