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. Author manuscript; available in PMC: 2013 Jul 5.
Published in final edited form as: Virology. 2012 Apr 21;428(2):121–127. doi: 10.1016/j.virol.2012.03.012

Fig. 2.

Fig. 2

Breadth of Env-specific T lymphocyte responses in the 12 individual monkeys from each experimental group, showing number of peptide epitopes recognized per protein. (A) The breadth of the vaccine-elicited cellular immune responses was determined by assessing peripheral blood T lymphocyte recognition of 5 different envelope sequences using a peptide/IFN-γ ELISpot assay. PBL from each vaccinated monkey were evaluated for IFN-γ ELISpot responses to pools of 15-mers overlapping by 11 amino acids spanning each of the 5 selected envelope sequences. Epitopes of each of the 5 indicator envelope proteins recognized by PBL of each of the experimentally vaccinated monkeys were enumerated by matrix mapping and confirmation with individual peptides. If two overlapping peptides gave a positive response, it was counted as a single response. The minimum numbers of possible T cell responses that can recognize each strain are shown. (B) A box plot showing the distribution of numbers of vaccine responses per strain elicited by the different vaccines.