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. Author manuscript; available in PMC: 2013 Feb 26.
Published in final edited form as: Amino Acids. 2011 Sep 11;42(2-3):549–558. doi: 10.1007/s00726-011-1032-x

Fig. 3.

Fig. 3

In vitro Characterization of AT6.1 shAZI Stable Cell Lines

a Rat AT6.1 cells stably expressing control (pRS, shGFP) or AZI shRNA (shAZI H83, shAZI M35) were analyzed by western blot for AZI levels. shAZI H83 cells had the greatest degree of knockdown, and slight knockdown was also observed with shAZI M35. No substantial changes were observed in AZ levels. b qRT-PCR analysis confirms decreased AZI levels in both shAZI cell lines compared to shGFP control cells (ANOVA, p=.044). c Following 6 days of growth in culture, AT6.1 shAZI H83 cells had approximately a 24% reduced rate of cell growth compared to control cell lines, while a 17% decrease was observed in shAZI M35 cells. (ANOVA, p=.042)