Fig. 4.
Knockdown of AZI in PC3M-LN4 Cells Decreases Tumor Growth in vivo
a Human prostate cancer cells stably expressing either control shGFP or shAZI were injected subcutaneously into nude mice. 1×106 cells were injected in a 1:1 mix with Matrigel, and ten animals were used for each cell line. Calipers were used to measure tumor size twice per week for two months, and tumor volume was calculated as previously described. As shown above, knockdown of AZI with shAZI H81 led to a 56% decrease in tumor growth in vivo, and knockdown with shAZI H84 resulted in a 44% decrease in tumor growth compared to control cells. b At the end of the experiment, tumors from each group were excised and weighed. Decreased growth of shAZI tumors was also detected as a decrease in end-tumor weight. shAZI H81 tumors had significantly decreased tumor weights (*, p=.031) compared to shGFP control cells