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Enhanced cellular uptake of chitosan/alginate nanoparticles by DR5 targeting to HCT116. Incubation of DR5-targeted (A) or nude (B) nanoparticle (NP) suspensions (100 μg/mL formulation) for 60 min before fixation, cell nucleus demonstrated in blue and rhodamine 6G labeled nanoparticles in red. (C) Quantification of cellular rhodamine 6G content by fluorescence intensities in regions of interest (ROI) performed on cells in three fields of view using ImageJ software. ***P < 0.005.
