Shh promotes neuron survival and neurite outgrowth in HH20-25 SAGs cultured for 24 hours.
SAG explants cultured with vehicle (Control; A), 0.5 μg/ml Shh (B), 5 μg/ml Shh (C), 10 μM Cyclopamine (E), 0.5 μg/ml Shh plus 10 μM Cyclopamine (F), or 5 μg/ml Shh+ 10 μM Cyclopamine (G). SAG explants co-cultured with beads soaked in PBS (Control; D) or 2.5 μg/ml Shh (H). Scale bar = 200 μm. Quantification of the average pixel number for SAG explants (I), the percentage of TUNEL positive neurons (J) and the mitotic index (K). Quantification of neurite outgrowth for SAG-bead co-cultures (L). See legend to figure 1 for labeling conventions.
***p<0.0001 and *p<0.05 significantly different from controls.