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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Dev Neurobiol. 2012 Jul 20;72(9):1213–1228. doi: 10.1002/dneu.20988

Figure 8.

Figure 8

Purified human FGF2 does not affect neurite outgrowth in HH20-25 SAGs cultured for 24 hours.

Quantification of the average pixel number for SAG explants (A) and the percentage of caspase-3 positive DF1 cells (B). No significant difference in pixel number was found between control and 1-20 ng/ml FGF2 treated explants (A; ANOVA, p=0.7874). There were significantly fewer caspase-3 positive DF-1 cells in the presence of 1 and 10 ng/ml FGF2 compared to controls (B; ANOVA, p<0.0001), demonstrating that the purified FGF2 protein used in the SAG experiments is bioactive.