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Overview of the pen microfluidics fabrication process. (a) An ink mask is drawn on a COC chip surface. (b) Vapor-phase solvent exposure results in patterned growth of the COC surface by solvent swelling. (b) Bonding is realized by bringing the patterned surface into contact with a sealing layer, followed by solvent bonding using a desktop laminator. (d) The water-soluble ink masking layer remaining within the sealed microchannel is removed by pumping aqueous buffer through the channel.
