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. Author manuscript; available in PMC: 2013 Feb 26.
Published in final edited form as: Nature. 2011 Nov 27;480(7378):561–564. doi: 10.1038/nature10657

Figure 2. Functional data for modulation of the equilibrium.

Figure 2

a, The effects of the translation pH on read-through activity of MLV-PK S1–L2 turn mutants in vitro. The data show the positive effect of increased stacking in U38A, which is abrogated in U38C and correlates with in vivo activity (Supplementary Fig. 6c). Error bars indicate standard error (n = 3). b, Hill plots27 (R ≥ 0.99) to determine the pKa values of A17 in the indicated MLV-PK constructs. ΔT is the total chemical shift difference for the C2 carbon of A17 in PKinactive and PKactive and Δ is the difference between the C2 chemical shift at a given pH and the maximum chemical shift. c, In vivo read-through levels for the MLV-PK wild type (WTB) and the U38A:A29C mutant.