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. Author manuscript; available in PMC: 2013 Nov 2.
Published in final edited form as: J Mol Biol. 2012 Aug 23;423(4):528–539. doi: 10.1016/j.jmb.2012.08.008

Table 2.

Energetic contribution of the C-terminus on binding

βS
 γS
mol% POPS ΔGtrunc (kcal/mol) ΔΔGC-term (kcal/mol) ΔGC-trunc (kcal/mol) ΔΔGC-term (kcal/mol)
0 −5.46±0.08 1.15±0.14 −4.84±0.10 0.86±0.18
25 −6.15±0.15 1.59±0.19 −5.17±0.09 1.00±0.24
33 −5.76±0.08 0.71 ±0.09 −5.22±0.05 0.78±0.13
50 −6.54±0.15 1.27±0.12 −6.00±0.24 0.74±0.31
75 −7.79±0.09 1.83±0.12 −6.41±0.19 0.30±0.22

The energetic contribution was calculated as ΔΔGC-term=ΔGFL−ΔGtrunc with ΔΔGC-term>0 indicating that the C-terminus destabilizes protein–liposome interactions. ΔΔGC-term, energetic contribution of the C-terminal segment; ΔGtrunc, free energy of partitioning of the truncated constructs; ΔGFL, free energy of partitioning of the full-length proteins.