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. Author manuscript; available in PMC: 2013 Feb 26.
Published in final edited form as: Toxicol Appl Pharmacol. 2011 Mar 30;253(2):112–120. doi: 10.1016/j.taap.2011.03.015

Figure 8. Localization of Hsp27 and Hsp70 in caveolae.

Figure 8

Mouse keratinocyte skin constructs were exposed to CEES (300 µM) or control. After 24 hr, caveolar (Cav) and post-nuclear supernatant (PNS) subcellular fractions were isolated from the cells using sucrose density gradient centrifugation as described in the Materials and Methods. Upper panel: Cav and PNS fractions were assayed for Hsp27 and Hsp70 by Western blotting. The relative purity of the caveolar fractions was determined by Western blot analysis using caveolin-1 antibodies. Lower panel: Effect of CEEES on Hsp27 and Hsp70 expression in cav and PNS subcellular fractions isolated from control and CEES-treated mouse keratinocytes.