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. Author manuscript; available in PMC: 2013 Sep 21.
Published in final edited form as: Chem Biol. 2012 Sep 21;19(9):1175–1186. doi: 10.1016/j.chembiol.2012.07.018

Figure 5. Selective Blockade of MCL-1-mediated Suppression of BAX Activation by MIM1.

Figure 5

(A) BH3-only protein tBID directly activates BAX-mediated liposomal release, which is effectively suppressed by treatment with anti-apoptotic MCL-1ΔNΔC and BCL-XLΔC.

(B) MCL-1 SAHBD selectively inhibited MCL-1ΔNΔC suppression of tBID-induced BAX activation.

(C) ABT-737 selectively inhibited BCL-XLΔC suppression of tBID-induced BAX activation.

(D) The activity profile of MIM1 in the liposomal release assay mirrored the MCL-1 selectivity of MCL-1 SAHBD.

Liposomal assays were conducted in triplicate for each condition and repeated with an independent preparation of recombinant BAX with similar results.