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. 1992 Jul;5(3):238–247. doi: 10.1128/cmr.5.3.238

Laboratory methods for early detection of human immunodeficiency virus type 1 in newborns and infants.

A V Sison 1, J M Campos 1
PMCID: PMC358242  PMID: 1498766

Abstract

Cumulative data on serological testing of newborns and infants have shown that (i) maternal and newborn anti-HIV-1 IgG titers are high at delivery, which may explain the persistence of antibody in the infants of seropositive mothers; (ii) in some situations, serial HIV-1 antibody testing may identify infected infants; and (iii) detection of anti-HIV-1 IgA or IgM is specific for infection but the sensitivity of this assay may be compromised in certain situations, such as when infected infants are hypogammaglobulinemic or when the rise and fall of HIV-1-specific IgM synthesis following acute infection has been completed before delivery of the infant. Cumulative data on PCR, viral culture, and tests for antigen in newborns and infants have shown that (i) among all age groups, viral culture is probably the most specific test available for detection of HIV-1, as PCR and the p24 antigen test may (though rarely) give false-positive results; (ii) the sensitivity of these tests increases in the order of antigen, culture, and PCR, with relatively insensitive results in the first 3 months of life for all of these tests; (iii) the sensitivity of all of these tests improves and approximates 90 to 100% when infants over 6 months of age are tested; and (iv) data regarding the sensitivity, specificity, and usefulness of these virological assays in infants under 3 months of age are very scant and inconclusive.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allain J. P., Laurian Y., Paul D. A., Verroust F., Leuther M., Gazengel C., Senn D., Larrieu M. J., Bosser C. Long-term evaluation of HIV antigen and antibodies to p24 and gp41 in patients with hemophilia. Potential clinical importance. N Engl J Med. 1987 Oct 29;317(18):1114–1121. doi: 10.1056/NEJM198710293171804. [DOI] [PubMed] [Google Scholar]
  2. Anderson R. E., Lang W., Shiboski S., Royce R., Jewell N., Winkelstein W., Jr Use of beta 2-microglobulin level and CD4 lymphocyte count to predict development of acquired immunodeficiency syndrome in persons with human immunodeficiency virus infection. Arch Intern Med. 1990 Jan;150(1):73–77. [PubMed] [Google Scholar]
  3. Andiman W. A. Virologic and serologic aspects of human immunodeficiency virus infection in infants and children. Semin Perinatol. 1989 Feb;13(1):16–26. [PubMed] [Google Scholar]
  4. Barré-Sinoussi F., Chermann J. C., Rey F., Nugeyre M. T., Chamaret S., Gruest J., Dauguet C., Axler-Blin C., Vézinet-Brun F., Rouzioux C. Isolation of a T-lymphotropic retrovirus from a patient at risk for acquired immune deficiency syndrome (AIDS). Science. 1983 May 20;220(4599):868–871. doi: 10.1126/science.6189183. [DOI] [PubMed] [Google Scholar]
  5. Blanche S., Rouzioux C., Moscato M. L., Veber F., Mayaux M. J., Jacomet C., Tricoire J., Deville A., Vial M., Firtion G. A prospective study of infants born to women seropositive for human immunodeficiency virus type 1. HIV Infection in Newborns French Collaborative Study Group. N Engl J Med. 1989 Jun 22;320(25):1643–1648. doi: 10.1056/NEJM198906223202502. [DOI] [PubMed] [Google Scholar]
  6. Borkowsky W., Krasinski K., Paul D., Holzman R., Moore T., Bebenroth D., Lawrence R., Chandwani S. Human immunodeficiency virus type 1 antigenemia in children. J Pediatr. 1989 Jun;114(6):940–945. doi: 10.1016/s0022-3476(89)80434-2. [DOI] [PubMed] [Google Scholar]
  7. Borkowsky W., Krasinski K., Paul D., Moore T., Bebenroth D., Chandwani S. Human-immunodeficiency-virus infections in infants negative for anti-HIV by enzyme-linked immunoassay. Lancet. 1987 May 23;1(8543):1168–1171. doi: 10.1016/s0140-6736(87)92143-x. [DOI] [PubMed] [Google Scholar]
  8. Brandt C. D., Rakusan T. A., Sison A. V., Josephs S. H., Saxena E. S., Herzog K. D., Parrott R. H., Sever J. L. Detection of human immunodeficiency virus type 1 infection in young pediatric patients by using polymerase chain reaction and biotinylated probes. J Clin Microbiol. 1992 Jan;30(1):36–40. doi: 10.1128/jcm.30.1.36-40.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Celum C. L., Coombs R. W., Lafferty W., Inui T. S., Louie P. H., Gates C. A., McCreedy B. J., Egan R., Grove T., Alexander S. Indeterminate human immunodeficiency virus type 1 western blots: seroconversion risk, specificity of supplemental tests, and an algorithm for evaluation. J Infect Dis. 1991 Oct;164(4):656–664. doi: 10.1093/infdis/164.4.656. [DOI] [PubMed] [Google Scholar]
  10. Chadwick E. G., Yogev R., Kwok S., Sninsky J. J., Kellogg D. E., Wolinsky S. M. Enzymatic amplification of the human immunodeficiency virus in peripheral blood mononuclear cells from pediatric patients. J Infect Dis. 1989 Dec;160(6):954–959. doi: 10.1093/infdis/160.6.954. [DOI] [PubMed] [Google Scholar]
  11. Chan M. M., Campos J. M., Josephs S., Rifai N. Beta 2-microglobulin and neopterin: predictive markers for human immunodeficiency virus type 1 infection in children? J Clin Microbiol. 1990 Oct;28(10):2215–2219. doi: 10.1128/jcm.28.10.2215-2219.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Coombs R. W., Collier A. C., Allain J. P., Nikora B., Leuther M., Gjerset G. F., Corey L. Plasma viremia in human immunodeficiency virus infection. N Engl J Med. 1989 Dec 14;321(24):1626–1631. doi: 10.1056/NEJM198912143212402. [DOI] [PubMed] [Google Scholar]
  13. Davey R. T., Jr, Lane H. C. Laboratory methods in the diagnosis and prognostic staging of infection with human immunodeficiency virus type 1. Rev Infect Dis. 1990 Sep-Oct;12(5):912–930. doi: 10.1093/clinids/12.5.912. [DOI] [PubMed] [Google Scholar]
  14. De Rossi A., Ades A. E., Mammano F., Del Mistro A., Amadori A., Giaquinto C., Chieco-Bianchi L. Antigen detection, virus culture, polymerase chain reaction, and in vitro antibody production in the diagnosis of vertically transmitted HIV-1 infection. AIDS. 1991 Jan;5(1):15–20. doi: 10.1097/00002030-199101000-00002. [DOI] [PubMed] [Google Scholar]
  15. De Rossi A., Amadori A., Chieco-Bianchi L., Giacquinto C., Zacchello F., Buchbinder A., Wong-Staal F., Gallo R. C., Peckham C. S. Polymerase chain reaction and in-vitro antibody production for early diagnosis of paediatric HIV infection. Lancet. 1988 Jul 30;2(8605):278–278. doi: 10.1016/s0140-6736(88)92565-2. [DOI] [PubMed] [Google Scholar]
  16. Edwards J. R., Ulrich P. P., Weintrub P. S., Cowan M. J., Levy J. A., Wara D. W., Vyas G. N. Polymerase chain reaction compared with concurrent viral cultures for rapid identification of human immunodeficiency virus infection among high-risk infants and children. J Pediatr. 1989 Aug;115(2):200–203. doi: 10.1016/s0022-3476(89)80065-4. [DOI] [PubMed] [Google Scholar]
  17. Ellerbrock T. V., Bush T. J., Chamberland M. E., Oxtoby M. J. Epidemiology of women with AIDS in the United States, 1981 through 1990. A comparison with heterosexual men with AIDS. JAMA. 1991 Jun 12;265(22):2971–2975. [PubMed] [Google Scholar]
  18. Embury S. H., Scharf S. J., Saiki R. K., Gholson M. A., Golbus M., Arnheim N., Erlich H. A. Rapid prenatal diagnosis of sickle cell anemia by a new method of DNA analysis. N Engl J Med. 1987 Mar 12;316(11):656–661. doi: 10.1056/NEJM198703123161103. [DOI] [PubMed] [Google Scholar]
  19. Epstein L. G., Boucher C. A., Morrison S. H., Connor E. M., Oleske J. M., Lange J. M., van der Noordaa J., Bakker M., Dekker J., Scherpbier H. Persistent human immunodeficiency virus type 1 antigenemia in children correlates with disease progression. Pediatrics. 1988 Dec;82(6):919–924. [PubMed] [Google Scholar]
  20. Escaich S., Wallon M., Baginski I., Ritter J., Philippe N., Bertrand Y., Claris O., Raudrant D., Sepetjan M., Trepo C. Comparison of HIV detection by virus isolation in lymphocyte cultures and molecular amplification of HIV DNA and RNA by PCR in offspring of seropositive mothers. J Acquir Immune Defic Syndr. 1991;4(2):130–135. [PubMed] [Google Scholar]
  21. Falloon J., Eddy J., Wiener L., Pizzo P. A. Human immunodeficiency virus infection in children. J Pediatr. 1989 Jan;114(1):1–30. doi: 10.1016/s0022-3476(89)80596-7. [DOI] [PubMed] [Google Scholar]
  22. Fleming D. W., Cochi S. L., Steece R. S., Hull H. F. Acquired immunodeficiency syndrome in low-incidence areas. How safe is unsafe sex? JAMA. 1987 Aug 14;258(6):785–787. [PubMed] [Google Scholar]
  23. Fox R. I., Dotan I., Compton T., Fei H. M., Hamer M., Saito I. Use of DNA amplification methods for clinical diagnosis in autoimmune diseases. J Clin Lab Anal. 1989;3(6):378–387. doi: 10.1002/jcla.1860030611. [DOI] [PubMed] [Google Scholar]
  24. Fuchs D., Spira T. J., Hausen A., Reibnegger G., Werner E. R., Felmayer G. W., Wachter H. Neopterin as a predictive marker for disease progression in human immunodeficiency virus type 1 infection. Clin Chem. 1989 Aug;35(8):1746–1749. [PubMed] [Google Scholar]
  25. Gaetano C., Scano G., Carbonari M., Giannini G., Mezzaroma I., Aiuti F., Marolla L., Casadei A. M., Carapella E. Delayed and defective anti-HIV IgM response in infants. Lancet. 1987 Mar 14;1(8533):631–631. doi: 10.1016/s0140-6736(87)90272-8. [DOI] [PubMed] [Google Scholar]
  26. Gallo R. C., Salahuddin S. Z., Popovic M., Shearer G. M., Kaplan M., Haynes B. F., Palker T. J., Redfield R., Oleske J., Safai B. Frequent detection and isolation of cytopathic retroviruses (HTLV-III) from patients with AIDS and at risk for AIDS. Science. 1984 May 4;224(4648):500–503. doi: 10.1126/science.6200936. [DOI] [PubMed] [Google Scholar]
  27. Greer C. E., Peterson S. L., Kiviat N. B., Manos M. M. PCR amplification from paraffin-embedded tissues. Effects of fixative and fixation time. Am J Clin Pathol. 1991 Feb;95(2):117–124. doi: 10.1093/ajcp/95.2.117. [DOI] [PubMed] [Google Scholar]
  28. Gwinn M., Pappaioanou M., George J. R., Hannon W. H., Wasser S. C., Redus M. A., Hoff R., Grady G. F., Willoughby A., Novello A. C. Prevalence of HIV infection in childbearing women in the United States. Surveillance using newborn blood samples. JAMA. 1991 Apr 3;265(13):1704–1708. [PubMed] [Google Scholar]
  29. Harper M. E., Marselle L. M., Gallo R. C., Wong-Staal F. Detection of lymphocytes expressing human T-lymphotropic virus type III in lymph nodes and peripheral blood from infected individuals by in situ hybridization. Proc Natl Acad Sci U S A. 1986 Feb;83(3):772–776. doi: 10.1073/pnas.83.3.772. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Ho D. D., Moudgil T., Alam M. Quantitation of human immunodeficiency virus type 1 in the blood of infected persons. N Engl J Med. 1989 Dec 14;321(24):1621–1625. doi: 10.1056/NEJM198912143212401. [DOI] [PubMed] [Google Scholar]
  31. Ho D. D., Rota T. R., Hirsch M. S. Infection of monocyte/macrophages by human T lymphotropic virus type III. J Clin Invest. 1986 May;77(5):1712–1715. doi: 10.1172/JCI112491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Jackson J. B., Balfour H. H., Jr Practical diagnostic testing for human immunodeficiency virus. Clin Microbiol Rev. 1988 Jan;1(1):124–138. doi: 10.1128/cmr.1.1.124. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Jackson J. B., Kwok S. Y., Sninsky J. J., Hopsicker J. S., Sannerud K. J., Rhame F. S., Henry K., Simpson M., Balfour H. H., Jr Human immunodeficiency virus type 1 detected in all seropositive symptomatic and asymptomatic individuals. J Clin Microbiol. 1990 Jan;28(1):16–19. doi: 10.1128/jcm.28.1.16-19.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Johnson J. P., Nair P., Hines S. E., Seiden S. W., Alger L., Revie D. R., O'Neil K. M., Hebel R. Natural history and serologic diagnosis of infants born to human immunodeficiency virus-infected women. Am J Dis Child. 1989 Oct;143(10):1147–1153. doi: 10.1001/archpedi.1989.02150220037017. [DOI] [PubMed] [Google Scholar]
  35. Krilov L. R., Kamani N., Hendry R. M., Wittek A. E., Quinnan G. V., Jr Longitudinal serologic evaluation of an infant with acquired immunodeficiency syndrome. Pediatr Infect Dis J. 1987 Nov;6(11):1066–1067. [PubMed] [Google Scholar]
  36. Krivine A., Yakudima A., Le May M., Pena-Cruz V., Huang A. S., McIntosh K. A comparative study of virus isolation, polymerase chain reaction, and antigen detection in children of mothers infected with human immunodeficiency virus. J Pediatr. 1990 Mar;116(3):372–376. doi: 10.1016/s0022-3476(05)82823-9. [DOI] [PubMed] [Google Scholar]
  37. Kühnl P., Seidl S., Holzberger G. HLA DR4 antibodies cause positive HTLV-III antibody ELISA results. Lancet. 1985 May 25;1(8439):1222–1223. doi: 10.1016/s0140-6736(85)92910-1. [DOI] [PubMed] [Google Scholar]
  38. Lai-Goldman M., Lai E., Grody W. W. Detection of human immunodeficiency virus (HIV) infection in formalin-fixed, paraffin-embedded tissues by DNA amplification. Nucleic Acids Res. 1988 Aug 25;16(16):8191–8191. doi: 10.1093/nar/16.16.8191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Landesman S., Weiblen B., Mendez H., Willoughby A., Goedert J. J., Rubinstein A., Minkoff H., Moroso G., Hoff R. Clinical utility of HIV-IgA immunoblot assay in the early diagnosis of perinatal HIV infection. JAMA. 1991 Dec 25;266(24):3443–3446. [PubMed] [Google Scholar]
  40. Laure F., Courgnaud V., Rouzioux C., Blanche S., Veber F., Burgard M., Jacomet C., Griscelli C., Brechot C. Detection of HIV1 DNA in infants and children by means of the polymerase chain reaction. Lancet. 1988 Sep 3;2(8610):538–541. doi: 10.1016/s0140-6736(88)92659-1. [DOI] [PubMed] [Google Scholar]
  41. Lee F. K., Nahmias A. J., Lowery S., Nesheim S., Reef S., Thompson S., Oleske J., Vahlne A., Czerkinsky C. ELISPOT: a new approach to studying the dynamics of virus-immune system interaction for diagnosis and monitoring of HIV infection. AIDS Res Hum Retroviruses. 1989 Oct;5(5):517–523. doi: 10.1089/aid.1989.5.517. [DOI] [PubMed] [Google Scholar]
  42. Levy J. A., Shimabukuro J. Recovery of AIDS-associated retroviruses from patients with AIDS or AIDS-related conditions and from clinically healthy individuals. J Infect Dis. 1985 Oct;152(4):734–738. doi: 10.1093/infdis/152.4.734. [DOI] [PubMed] [Google Scholar]
  43. Lewis S. H., Reynolds-Kohler C., Fox H. E., Nelson J. A. HIV-1 in trophoblastic and villous Hofbauer cells, and haematological precursors in eight-week fetuses. Lancet. 1990 Mar 10;335(8689):565–568. doi: 10.1016/0140-6736(90)90349-a. [DOI] [PubMed] [Google Scholar]
  44. Lyman W. D., Kress Y., Kure K., Rashbaum W. K., Rubinstein A., Soeiro R. Detection of HIV in fetal central nervous system tissue. AIDS. 1990 Sep;4(9):917–920. doi: 10.1097/00002030-199009000-00014. [DOI] [PubMed] [Google Scholar]
  45. Mano H., Chermann J. C. Fetal human immunodeficiency virus type 1 infection of different organs in the second trimester. AIDS Res Hum Retroviruses. 1991 Jan;7(1):83–88. doi: 10.1089/aid.1991.7.83. [DOI] [PubMed] [Google Scholar]
  46. Martin N. L., Levy J. A., Legg H., Weintrub P. S., Cowan M. J., Wara D. W. Detection of infection with human immunodeficiency virus (HIV) type 1 in infants by an anti-HIV immunoglobulin A assay using recombinant proteins. J Pediatr. 1991 Mar;118(3):354–358. doi: 10.1016/s0022-3476(05)82146-8. [DOI] [PubMed] [Google Scholar]
  47. Mok J. Q., Giaquinto C., De Rossi A., Grosch-Wörner I., Ades A. E., Peckham C. S. Infants born to mothers seropositive for human immunodeficiency virus. Preliminary findings from a multicentre European study. Lancet. 1987 May 23;1(8543):1164–1168. doi: 10.1016/s0140-6736(87)92142-8. [DOI] [PubMed] [Google Scholar]
  48. Monroe J. E., Andrews C., Sullivan J. L., Mulder C. Use of cytoplasmic dot-blot hybridization to detect human immunodeficiency virus RNA sequences in cultures of peripheral blood. J Infect Dis. 1987 Feb;155(2):320–322. doi: 10.1093/infdis/155.2.320. [DOI] [PubMed] [Google Scholar]
  49. Mullis K. B., Faloona F. A. Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods Enzymol. 1987;155:335–350. doi: 10.1016/0076-6879(87)55023-6. [DOI] [PubMed] [Google Scholar]
  50. Mullis K., Faloona F., Scharf S., Saiki R., Horn G., Erlich H. Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction. Cold Spring Harb Symp Quant Biol. 1986;51(Pt 1):263–273. doi: 10.1101/sqb.1986.051.01.032. [DOI] [PubMed] [Google Scholar]
  51. Mundy D. C., Schinazi R. F., Gerber A. R., Nahmias A. J., Randall H. W., Jr Human immunodeficiency virus isolated from amniotic fluid. Lancet. 1987 Aug 22;2(8556):459–460. doi: 10.1016/s0140-6736(87)91001-4. [DOI] [PubMed] [Google Scholar]
  52. Nishanian P., Huskins K. R., Stehn S., Detels R., Fahey J. L. A simple method for improved assay demonstrates that HIV p24 antigen is present as immune complexes in most sera from HIV-infected individuals. J Infect Dis. 1990 Jul;162(1):21–28. doi: 10.1093/infdis/162.1.21. [DOI] [PubMed] [Google Scholar]
  53. Pahwa S., Chirmule N., Leombruno C., Lim W., Harper R., Bhalla R., Pahwa R., Nelson R. P., Good R. A. In vitro synthesis of human immunodeficiency virus-specific antibodies in peripheral blood lymphocytes of infants. Proc Natl Acad Sci U S A. 1989 Oct;86(19):7532–7536. doi: 10.1073/pnas.86.19.7532. [DOI] [PMC free article] [PubMed] [Google Scholar]
  54. Paterlini P., Lallemant-Le Coeur S., Lallemant M., M'Pelé P., Dazza M. C., Terre S., Moncany M., Jourdain G., Courgnaud V., N'Zingoula S. Polymerase chain reaction for studies of mother to child transmission of HIV1 in Africa. J Med Virol. 1990 Jan;30(1):53–57. doi: 10.1002/jmv.1890300112. [DOI] [PubMed] [Google Scholar]
  55. Paul D. A., Falk L. A., Kessler H. A., Chase R. M., Blaauw B., Chudwin D. S., Landay A. L. Correlation of serum HIV antigen and antibody with clinical status in HIV-infected patients. J Med Virol. 1987 Aug;22(4):357–363. doi: 10.1002/jmv.1890220408. [DOI] [PubMed] [Google Scholar]
  56. Peuchmaur M., Delfraissy J. F., Pons J. C., Emilie D., Vazeux R., Rouzioux C., Brossard Y., Papiernik E. HIV proteins absent from placentas of 75 HIV-1-positive women studied by immunohistochemistry. AIDS. 1991 Jun;5(6):741–745. doi: 10.1097/00002030-199106000-00015. [DOI] [PubMed] [Google Scholar]
  57. Pomerantz R. J., de la Monte S. M., Donegan S. P., Rota T. R., Vogt M. W., Craven D. E., Hirsch M. S. Human immunodeficiency virus (HIV) infection of the uterine cervix. Ann Intern Med. 1988 Mar;108(3):321–327. doi: 10.7326/0003-4819-108-3-321. [DOI] [PubMed] [Google Scholar]
  58. Pyun K. H., Ochs H. D., Dufford M. T., Wedgwood R. J. Perinatal infection with human immunodeficiency virus. Specific antibody responses by the neonate. N Engl J Med. 1987 Sep 3;317(10):611–614. doi: 10.1056/NEJM198709033171006. [DOI] [PubMed] [Google Scholar]
  59. Quinn T. C., Kline R. L., Halsey N., Hutton N., Ruff A., Butz A., Boulos R., Modlin J. F. Early diagnosis of perinatal HIV infection by detection of viral-specific IgA antibodies. JAMA. 1991 Dec 25;266(24):3439–3442. [PubMed] [Google Scholar]
  60. Ragni M. V., Urbach A. H., Taylor S., Claassen D., Gupta P., Lewis J. H., Ho D. D., Shaw G. M. Isolation of human immunodeficiency virus and detection of HIV DNA sequences in the brain of an ELISA antibody-negative child with acquired immune deficiency syndrome and progressive encephalopathy. J Pediatr. 1987 Jun;110(6):892–894. doi: 10.1016/s0022-3476(87)80404-3. [DOI] [PubMed] [Google Scholar]
  61. Rogers M. F., Ou C. Y., Kilbourne B., Schochetman G. Advances and problems in the diagnosis of human immunodeficiency virus infection in infants. Pediatr Infect Dis J. 1991 Jul;10(7):523–531. doi: 10.1097/00006454-199107000-00009. [DOI] [PubMed] [Google Scholar]
  62. Rogers M. F., Ou C. Y., Rayfield M., Thomas P. A., Schoenbaum E. E., Abrams E., Krasinski K., Selwyn P. A., Moore J., Kaul A. Use of the polymerase chain reaction for early detection of the proviral sequences of human immunodeficiency virus in infants born to seropositive mothers. New York City Collaborative Study of Maternal HIV Transmission and Montefiore Medical Center HIV Perinatal Transmission Study Group. N Engl J Med. 1989 Jun 22;320(25):1649–1654. doi: 10.1056/NEJM198906223202503. [DOI] [PubMed] [Google Scholar]
  63. Ryder R. W., Nsa W., Hassig S. E., Behets F., Rayfield M., Ekungola B., Nelson A. M., Mulenda U., Francis H., Mwandagalirwa K. Perinatal transmission of the human immunodeficiency virus type 1 to infants of seropositive women in Zaire. N Engl J Med. 1989 Jun 22;320(25):1637–1642. doi: 10.1056/NEJM198906223202501. [DOI] [PubMed] [Google Scholar]
  64. Schnittman S. M., Greenhouse J. J., Psallidopoulos M. C., Baseler M., Salzman N. P., Fauci A. S., Lane H. C. Increasing viral burden in CD4+ T cells from patients with human immunodeficiency virus (HIV) infection reflects rapidly progressive immunosuppression and clinical disease. Ann Intern Med. 1990 Sep 15;113(6):438–443. doi: 10.7326/0003-4819-113-6-438. [DOI] [PubMed] [Google Scholar]
  65. Schüpbach J., Wunderli W., Kind C., Kernen R., Baumgartner A., Tomasik Z. Frequent detection of HIV- and IgG-specific IgM and IgA antibodies in HIV-positive cord-blood sera: fine analysis by western blot. AIDS. 1989 Sep;3(9):583–589. [PubMed] [Google Scholar]
  66. Scott G. B., Buck B. E., Leterman J. G., Bloom F. L., Parks W. P. Acquired immunodeficiency syndrome in infants. N Engl J Med. 1984 Jan 12;310(2):76–81. doi: 10.1056/NEJM198401123100202. [DOI] [PubMed] [Google Scholar]
  67. Shaw G. M., Hahn B. H., Arya S. K., Groopman J. E., Gallo R. C., Wong-Staal F. Molecular characterization of human T-cell leukemia (lymphotropic) virus type III in the acquired immune deficiency syndrome. Science. 1984 Dec 7;226(4679):1165–1171. doi: 10.1126/science.6095449. [DOI] [PubMed] [Google Scholar]
  68. Smith D. M., Dewhurst S., Shepherd S., Volsky D. J., Goldsmith J. C. False-positive enzyme-linked immunosorbent assay reactions for antibody to human immunodeficiency virus in a population of midwestern patients with congenital bleeding disorders. Transfusion. 1987 Jan-Feb;27(1):112–112. doi: 10.1046/j.1537-2995.1987.27187121453.x. [DOI] [PubMed] [Google Scholar]
  69. Spadoro J. P., Payne H., Lee Y., Rosenstraus M. J. Single copies of HIV proviral DNA detected by fluorescent in situ hybridization. Biotechniques. 1990 Aug;9(2):186–195. [PubMed] [Google Scholar]
  70. Sperling R. S., Sacks H. S., Mayer L., Joyner M., Berkowitz R. L. Umbilical cord blood serosurvey for human immunodeficiency virus in parturient women in a voluntary hospital in New York City. Obstet Gynecol. 1989 Feb;73(2):179–181. [PubMed] [Google Scholar]
  71. Vendrell J. P., Segondy M., Ducos J., Reynes J., Huguet M. F., Nicolas J. C., Serre A. Analysis of the spontaneous in vitro anti-HIV-1 antibody secretion by peripheral blood mononuclear cells in HIV-1 infection. Clin Exp Immunol. 1991 Feb;83(2):197–202. doi: 10.1111/j.1365-2249.1991.tb05614.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  72. Wages J. M., Jr, Hamdallah M., Calabro M. A., Fowler A. K., Oster C. N., Redfield R. R., Burke D. S. Clinical performance of a polymerase chain reaction testing algorithm for diagnosis of HIV-1 infection in peripheral blood mononuclear cells. J Med Virol. 1991 Jan;33(1):58–63. doi: 10.1002/jmv.1890330112. [DOI] [PubMed] [Google Scholar]
  73. Weiblen B. J., Lee F. K., Cooper E. R., Landesman S. H., McIntosh K., Harris J. A., Nesheim S., Mendez H., Pelton S. I., Nahmias A. J. Early diagnosis of HIV infection in infants by detection of IgA HIV antibodies. Lancet. 1990 Apr 28;335(8696):988–990. doi: 10.1016/0140-6736(90)91061-e. [DOI] [PubMed] [Google Scholar]
  74. Weiblen B. J., Schumacher R. T., Hoff R. Detection of IgM and IgA HIV antibodies after removal of IgG with recombinant protein G. J Immunol Methods. 1990 Feb 9;126(2):199–204. doi: 10.1016/0022-1759(90)90151-k. [DOI] [PubMed] [Google Scholar]
  75. Weintrub P. S., Ulrich P. P., Edwards J. R., Boucher F., Levy J. A., Cowan M. J., Vyas G. N. Use of polymerase chain reaction for the early detection of HIV infection in the infants of HIV-seropositive women. AIDS. 1991 Jul;5(7):881–884. doi: 10.1097/00002030-199107000-00014. [DOI] [PubMed] [Google Scholar]
  76. Williams P., Simmonds P., Yap P. L., Balfe P., Bishop J., Brettle R., Hague R., Hargreaves D., Inglis J., Brown A. L. The polymerase chain reaction in the diagnosis of vertically transmitted HIV infection. AIDS. 1990 May;4(5):393–398. doi: 10.1097/00002030-199005000-00003. [DOI] [PubMed] [Google Scholar]

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