Table 1.
Summary of the percentage of cells expressing selected cytokines as determined by flow cytometry. Response (fold increase) of CD4+ and CD8+ lymphocytes from rBCG-immunized mice (rBCG018, rBCG032) was compared to those of BCG-immunized mice (BCG).
| CD4+/IL-2 | Ag85B (P1) | Ag85B (P2) | Ag85B (P3) | Mtb8.4 | ESAT-6 | CFP10 | MTP40 (P1) | MTP40 (P2) |
|---|---|---|---|---|---|---|---|---|
| BCG rBCG018 rBCG032 |
6.7 11.5 12.5 |
7.4 20.6* 16.5* |
4.8 11.4* 6.5 |
3.8 12.5** 8.6* |
6.1 3.6 5.6 |
3.1 2.4 8.4* |
4.6 3.3 6.4 |
6.3 4.5 5.3 |
| CD4+/IL-4 | ||||||||
| BCG rBCG018 rBCG032 |
8.5 10.1 10.4 |
9.3 7.7 15.8 |
8.5 16.9 18.4* |
9.4 8.4 13.8 |
3.5 1.1 3.8 |
5.4 3.5 5.7 |
8.4 7.8 8.6 |
5.9 5.7 8.4 |
| CD4+/IFN-γ | ||||||||
| BCG rBCG018 rBCG032 |
4.6 17.4** 18.4** |
8.0 21.5* 22.5* |
6.4 16.6* 8.4 |
3.7 8.9* 7.5* |
5.4 5.5 7.4 |
5.7 2.5 5.7 |
5.6 2.7 9.0 |
3.8 2.7 9.7* |
| CD8+/IL-2 | ||||||||
| BCG rBCG018 rBCG032 |
12.3 21.5 25.0* |
9.4 34.3** 30.5** |
2.1 36.3** 30.4** |
2.7 10.4** 9.8** |
5.3 5.2 19.4** |
3.6 6.4 18.3** |
9.4 8.7 19.3* |
4.7 5.5 7.4 |
| CD8+/IL-4 | ||||||||
| BCG rBCG018 rBCG032 |
5.5 16.5** 20.8** |
3.7 15.5** 12.5** |
7.8 16.5* 20.5* |
5.7 8.4 13.8* |
4.8 7.5 13.8* |
4.6 8.6 11.4* |
6.4 8.0 11.5 |
6.0 7.3 7.4 |
| CD8+/IFN-γ | ||||||||
| BCG rBCG018 rBCG032 |
5.5 20.5** 25.4** |
8.7 20.5* 24.5* |
6.5 19.5** 21.5** |
3.8 10.5* 9.7* |
9.7 13.2 15.4 |
4.7 10.4* 11.3* |
8.4 9.9 14.3 |
5.9 7.7 9.5 |
** ≥ 3 fold increase (high response) * ≥ 2 fold increase (moderate response) ≤ 2 fold increase (weak to no response)