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. 2013 Feb 25;14(Suppl 1):S8. doi: 10.1186/1471-2172-14-S1-S8

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Copyright ©2013 Fernández et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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B. pertussis PL composition analysis; Panel A, SDS-PAGE (polyacrylamide 13 %) followed by R250 Blue Coomasie stain. Lane 1: Low molecular weight markers (97-14.4 kDa), Lane 2: B. pertussis PL. Panel B, SDS-PAGE of B. pertussis PL (polyacrylamide 13 %) followed by a LPS-specific silver stain. Panel C, Western blot of B. pertussis PL using Mabs or serum vs relevant antigens; Lane 1: Low molecular weight markers (97-14.4 kDa), Lane 2: PT anti serum (97/572), Lane 3: FIM 3 Mab (04/156), Lane 4: PRN anti serum (97/558).