Table 2.
Leader sequences in mRNA4 | Protein encoded by RNA31 | Genotype of RNA 1 and 22 | Replicating (R) or inducible(I) | Name of combined CMV variants |
---|---|---|---|---|
wt |
CP |
Subgroup I |
R |
CMVarI wt |
2 |
|
|
|
CMVarI 2 |
6 |
|
|
|
CMVarI 6 |
8 |
|
|
|
CMVarI 8 |
wt |
CP |
Subgroup II |
R |
CMVarII wt |
2 |
|
|
|
CMVarII 2 |
6 |
|
|
|
CMVarII 6 |
8 |
|
|
|
CMVarII 8 |
wt |
CP |
Subgroup II |
I |
CMVinII wt |
2 |
|
|
|
CMVinII 2 |
6 |
|
|
|
CMVinII 6 |
8 |
|
|
|
CMVinII 8 |
wt |
GFP |
Subgroup I |
R |
CMVarI Gwt |
2 |
|
|
|
CMVarI G2 |
6 |
|
|
|
CMVarI G6 |
8 |
|
|
|
CMVarI G8 |
wt |
GFP |
Subgroup II |
R |
CMVarII Gwt |
2 |
|
|
|
CMVarII G2 |
6 |
|
|
|
CMVarII G6 |
8 |
|
|
|
CMVarII G8 |
wt |
GFP |
Subgroup II |
I |
CMVinII Gwt |
2 |
|
|
|
CMVinII G2 |
6 |
|
|
|
CMVinII G6 |
8 |
|
|
|
CMVinII G8 |
wt |
E1 |
Subgroup I |
R |
CMVarI Ewt |
2 |
|
|
|
CMVarI E2 |
6 |
|
|
|
CMVarI E6 |
8 |
|
|
|
CMVarI E8 |
wt |
E1 |
Subgroup II |
R |
CMVarII Ewt |
2 |
|
|
|
CMVarII E2 |
6 |
|
|
|
CMVarII E6 |
8 |
|
|
|
CMVarII E8 |
wt |
E1 |
Subgroup II |
I |
CMVinII Ewt |
2 |
|
|
|
CMVinII E2 |
6 |
|
|
|
CMVinII E6 |
8 |
|
|
|
CMVinII E8 |
wt |
GFP |
Subgroup I |
R |
CMVarI 33Gwt |
2 |
|
|
|
CMVarI 33 G2 |
6 |
|
|
|
CMVarI 33 G6 |
8 |
|
|
|
CMVarI 33 G8 |
wt |
GFP |
Subgroup II |
R |
CMVarII 33Gwt |
2 |
|
|
|
CMVarII 33 G2 |
6 |
|
|
|
CMVarII 33 G6 |
8 |
|
|
|
CMVarII 33 G8 |
wt |
GFP |
Subgroup II |
I |
CMVinII 33Gwt |
2 |
|
|
|
CMVinII 33 G2 |
6 |
|
|
|
CMVinII 33 G6 |
8 |
|
|
|
CMVinII 33 G8 |
wt |
E1 |
Subgroup I |
R |
CMVarI 33Ewt |
2 |
|
|
|
CMVarI 33E2 |
6 |
|
|
|
CMVarI 33E6 |
8 |
|
|
|
CMVarI 33E8 |
wt |
E1 |
Subgroup II |
R |
CMVarII 33Ewt |
2 |
|
|
|
CMVarII 33E2 |
6 |
|
|
|
CMVarII 33E6 |
8 |
|
|
|
CMVarII 33E8 |
wt |
E1 |
Subgroup II |
I |
CMVinII 33Ewt |
2 |
|
|
|
CMVinII 33E2 |
6 |
|
|
|
CMVinII 33E6 |
8 | CMVinII 33E8 |
1All RNA3 constructs are from subgroup II CMV-Q. 2RNA 1 and 2 sources are subgroup I or subgroup II as indicated. Cells containing required plasmids were mixed and infiltrated into non-transgenic N. benthamiana plants as described.