. 2012 Sep 21;12:66. doi: 10.1186/1472-6750-12-66

Table 3.

Amount of active E1 (μg/g FW) in infiltrated N. benthamiana leaves using the different CMVin and CMVar leader sequence variants

	-wt	−2	−6	−8
CMVinII E	1.09 ± 0.20^1D	−0.01 ± 0.00^D	14.74 ± 2.14^A	13.97 ± 3.40^A
	1.07 ± 0.80^E	0.22 ± 0.36^E	8.76 ± 3.49^BC	9.38 ± 2.10^BC
	1.00 ± 0.45^D	0.76 ± 0.11^D	10.05 ± 1.28^B	11.05 ± 3.85^B
CMVarII E	2.23 ± 0.59^CD	0.00 ± 0.01^D	0.10 ± 0.08^D	−0.01 ± 0.00^D
	6.30 ± 3.50^CD	0.01 ± 0.03^E	0.33 ± 0.33^E	0.05 ± 0.12^E
	10.78 ± 3.63^BC	−0.01 ± 0.01^D	1.58 ± 0.49^D	1.85 ± 1.13^D
CMVarI E	4.12 ± 3.35^CD	0.00 ± 0.01^D	0.67 ± 0.36^D	−0.01 ± 0.00^D
	9.38 ± 2.10^AB	0.00 ± 0.01^E	0.57 ± 0.45^E	0.38 ± 0.79^E
	15.15 ± 5.39^AB	0.39 ± 0.30^D	1.88 ± 0.32^D	1.28 ± 0.53^D
CMVarII 33E	6.74 ± 2.82^BC	0.08 ± 0.12^D	1.17 ± 0.82^D	2.27 ± 1.81^D
	5.61 ± 2.01^CDE	0.01 ± 0.01^E	1.87 ± 0.64^DE	1.15 ± 0.77^DE
	14.06 ± 2.71^AB	0.21 ± 0.26^D	1.17 ± 0.98^CD	1.72 ± 0.93^CD
CMVarI 33E	11.34 ± 2.67^AB	1.42 ± 0.33^D	2.26 ± 0.36^CD	1.31 ± 0.81^CD
CMVarI 33E	19.60 ± 4.56^A	1.49 ± 0.14^DE	2.03 ± 0.31^CE	1.73 ± 1.09^DE
	20.99 ± 5.18^A	1.16 ± 0.96^D	1.97 ± 0.48^D	2.49 ± 1.61^D

¹Numbers indicate E1 production as μg/g fresh weight. Average ± standard deviations are shown. Samples were collected 6 days post-infiltration, using 3 plants, 2 leaves from each plant (n = 6) for each biological trial. Results for three different biological trials are shown for each test. The identities of variants tested are given in Table 2. Group superscript letters next to the numbers represent different statistical groups, means with the same letter are not significantly different by the Bonferroni (Dunn) t test using the SAS 9.1 program.