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. 2013 Feb 11;44(1):8. doi: 10.1186/1297-9716-44-8

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Copyright ©2013 Macchi et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Absolute numbers of thrombocytes (A) of chickens infected with Plasmodium gallinaceum, treated (black bars) or not (gray bars), with aminoguanidine and grouped by parasitemia range (PR). Thrombocytes of infected chickens stained with Giemsa in blood smears (B - D) and after culture for 1 h (E - H). Thrombocytes became larger and with more vacuoles when parasitemia was higher (B - D). (E) Normal thrombocytes with typical vacuoles (arrow). (F) Pyknotic thrombocytes. Note condensed chromatin (arrows). Atypical thrombocytes with long mononucleus (G) and two nuclei (arrows) (H) are seen. Bar = 10 μm. For the number of chickens by PR, number of experiments and significance of symbols, see Figure 6.